Abstract: Fracture prediction and evaluation is extremely important to shale oil exploration and development. Reliable fracture characterization can support the deployment and drilling of horizontal wells and facilitate optimal placement of fracturing operations, so as to form a network structure by connecting hydraulic fractures with natural fractures and thereby improve single-well EUR. Based on poststack and prestack seismic data combined with drilling data and regional geological background, this study focuses on multi-scale fracture prediction for the shale reservoirs of the second member of the Funing Formation in Huazhuang area. The results show that the high-definition ant body based on depth-domain seismic data effectively identifies medium- and large-scale fractures. Anisotropic detection using prestack seismic data effectively predicts small-scale fractures. Multi-scale fracture fusion clarifies the fracture development characteristics in the study area. The actual drilling, tracer, and imaging logging results in the study area further verify the accuracy and reliability of multi-scale fracture prediction, which provides technical support for the deployment of shale oil horizontal wells.